MORE | Spring 2024

Investigating the Effects of Oxygen Availability on Neurodevelopment in Organoid Models

Cortical organoids are three-dimensional structures by which neural development could be studied in vitro. However, they are limited in their ability to recreate the complex physiology and circuitry of the human brain, limiting their accuracy as models for disease and injury. This may occur due to the culturing conditions of organoids not being replicative of the human body. In this study, human induced pluripotent stem cells (iPSCs) were differentiated to cortical organoids and cultured for five weeks at the standard 20% oxygen level and a lower oxygen condition, mimicking a more accurate environment similar to the human brain.