FURI | Spring 2018

Progerin-Induced Aging to Develop a Human-Induced Pluripotent Stem Cell Model of Alzheimer’s Disease

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Alzheimer’s Disease (AD) has the sixth highest mortality rate in the United States with no known cure or treatment. To model late-onset disorders, such as AD, age-related phenotypes must be reintroduced into the DNA of the cells. The accumulation of the progerin protein is associated with aging. Using in-vitro techniques, it is hypothesized that overexpressing progerin through the generation of a lentiviral system will artificially age human induced pluripotent stem cell (hiPSC) derived neurons to model AD. Age-related phenotypes in hiPSCs will be analyzed via immunofluorescence (IF) microscopy and mitochondrial superoxide assays to confirm the identity of the hiPSCs.

Student researcher

Portrait of Frisch, Carlyle

Carlye Frisch

Biomedical engineering

Hometown: Scottsdale, Arizona

Graduation date: Spring 2020