FURI | Fall 2020

A Comparison of CRISPR/Cas-Derived Genome Editing Methods for hPSCs

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 systems are a vital gene editing tool for generating in vitro disease models. Since its debut, many CRISPR-derived editing techniques have been created to increase editing efficiency and fidelity. While these techniques all improve some aspects of functionality, there are insufficient resources for determining the optimal workflow for achieving different objectives in human pluripotent stem cells. This literature review compares the characteristics of four predominant editing agents (nucleases, base editors, transposases/recombinases, and prime editors) and discusses the appropriate utilization of each method.